The Accuris SmartReader™ Multimode is an advanced microplate reader designed to meet the growing needs of advanced life science labs. Equipped with a full suite of high-performance optical systems, the SmartReader Multimode is your all-in-one solution for absorbance, fluorescence and luminescence applications.
The SmartReader Multimode quickly processes assays in standard 96 well plates and can also accept 6, 12, 24, 48 and 384 well plates. Programming and operation are managed from the instrument’s large color touch screen and no external computer or software is required. Optional PC software is available for easy control and data handling via a Windows™ PC. The friendly and intuitive interface guides the user through various options for assay set up and processing. The instrument features a built-in plate shaker with linear, orbital, and double-orbital shaking modes, and an incubator that can perform top heating from ambient + 4°C – 45°C. Top heating creates a temperature gradient to prevent condesnation when lids are used on the microplates. Upon startup, the SmartReader Multimode performs a self-diagnostic test, followed by an automatic calibration of the entire system, ensuring consistent results across a wide range of assays.
Features
- Accepts standard 96-well plates, as well as 6, 12, 24, 48 and 384-well plates
- Monochromator-based system for UV through visible absorbance
- Filter module system for fluorescence assays
- Advanced PMT detector for luminescence assays
- Intuitive, 10-inch color touch screen
- Stand-alone system with optional PC software (FDA CFR Part 11)
- Monochromator-based system for UV through visible absorbance
- Filter module system for fluorescence assays
- Advanced PMT detector for luminescence assays
- Optional automatic injection modules for flash luminescence
UV-Visible Absorbance
Utilizing an advanced monochromator and a long-life, broad-spectrum xenon flash lamp, the SmartReader Multimode can produce incident light at user-selected wavelengths from 200 to 1000 nm in 1 nm steps. Processing assays is fast; a full 96 well plate can be read in under 15 seconds. The kinetic assay feature performs multiple readings over a set period of time, with curves for each well displayed in real-time. The optional SmartDrop™ plate allows fast and convenient UV absorbance evaluation of up to 16 nucleic acid and protein sample droplets.
Absorbance Applications
- ELISA Assays
- Colorimetrics Assays (Bradford, Lowry, etc.)
- Enzyme Kinetics
- Spectral Scanning
- DNA & RNA Analysis
- …and more
Fluorescence
Three filter modules are included to provide the most common excitation and emission wavelengths needed for fluorescence assays. Additional filter modules are available. These advanced modules incorporate dichroic mirrors with narrow band-pass optical filters and ensure high light transmission, increased detection sensitivity, and are easily exchanged. Modules are barcoded for automatic loading of wavelength data. The SmartReader Multimode allows for automatic dynamic range selection, which allows for the gain of the photomultiplier tube to accurately capture and normalize the signal intensity of samples.
Luminescence
The SmartReader Multimode is capable of luminescent detection, and can process both flash and glow assays. An advanced PMT (photo multiplier tube) enhances sensitivity of weak signals, prevents over-saturation of strong signals, and provides an ideal detection range for all common luminescence applications. Up to 2 sample injectors (available separately) can be installed and are compatible with both 96 and 384-well plates.
Data & Software
Data from the SmartReader Multimode to can be exported as either a .XLS or .CSV file via the included USB flash drive, the FTP server, or by using the optional SmartReader™ PC Software.
The SmartReader PC Software allows for remote programming & operation of the instrument via cable connection. Data from the instrument is automatically uploaded to the connected PC for further analyses. Both a Research version for academic use, and a FDA 21 CFR Part 11 compliant version are available.
- Measurement Modes:
- Absorbance, Fluorescence, Luminescence
- Plate Formats:
- 6 to 384 well plates
- Shaking:
- Linear (4.7, 7.0, 13.5 Hz), Orbital and double orbital (240-1200 rpm)
- Incubation Temperature Range:
- +4 °C to 45 °C
- Display:
- 10 in. touchscreen display
- Communication:
- 2 x USB A, USB B, Ethernet, RS232
- Absorbance Light Source:
- High energy Xenon flash lamp
- Absorbance Detector:
- Photodiode
- Absorbance Wavelength Accuracy:
- 2 nm
- Absorbance Wavelength Repeatability:
- 0.2 nm
- Absorbance Bandwidth (FWHM):
- > 2.5 nm
- Absorbance Wavelength Range:
- 200 – 1000 nm, 1nm steps
- Absorbance:
- 0.0 – 4.0 OD
- Absorbance Resolution:
- 0.0001 OD
- Absorbance Accuracy:
- 96-precision mode:± (1.0 %+0.003Abs)@ (0.0-2.0Abs) ;± 2.0 % @ (2.0-3.0Abs)
- Absorbance Repeatability:
- CV < 1.0 % or SD< 0.003 Fast(0.0-3.0Abs)CV < 0.5 % or SD< 0.003 Accurate(0.0-3.0Abs)
- Absorbance Stray Light:
- 0.1 % @ 220 nm
- Absorbance Linearity:
- R2 > 0.999 @ (0.0-3.0Abs)
- Absorbance Measurement Speed:
- < 15 sec.
- Fluorescence Reading Mode:
- Top reading
- Fluorescence Excitation Light Source:
- High energy Xenon flash lamp
- Fluorescence Detector:
- Photo Multiplier Tube (PMT)
- Fluorescence Detection Limit:
- ≤ 1 pM
- Fluorescence Wavelength Range:
- EX: 200 – 1000 nm; EM: 270 – 850 nm
- Fluorescence Filter Cartridges:
- (EX / EM): 470 / 525nm ; 523 / 564nm ; 624 / 692nm (included)
- Fluorescence Linear Dynamic Range:
- 6 logs
- Luminescence Detector:
- Photo Multiplier Tube (PMT)
- Luminescence Detection Limit:
- 15 amol / well
- Luminescence Linear Dynamic Range:
- 6 logs
- Power:
- 115V, 60Hz
- Dimensions (W x D x H):
- 16.5 x 21.7 x 15.2 in.
- Weight:
- 72.8 lbs
- Manufacturer:
- Accuris Instruments
- Model No.:
- MR9620
- Product Type:
- Microplate Reader
