Accuris SmartGlow LD Loading Dye
SmarGlow Pre Stain was developed as a safer, direct alternative for Ethidium Bromide (EtBr) in agarose and polyacrylamide gel electrophoresis. SmartGlow PS fluoresces green when bount to dsDNA or ssDNA while fluorescing red when bound to RNA. Excitation peaks between 290nm and 490nm, making it suitable to use with either UV or blue light.
Protocol for SmartGlow PS
- Prepare 100ml of agarose or polyacrylamide solution.
- Add 5ul of SmartGlow PS stain to the gel solution before pouring gels.
- For enhanced results, add SmartGlow PS to the running buffer at a ratio of 2.5ul to 5ul per 100ml. Adding SmartGlow PS to the running buffer will result in increased sensitivity and better detection of small quantities of nucleic acid.
- After electrophoresis is complete, view the gel using a UV or blue light illuminator
